-Abstract-
Development of periodontal tissues requires orchestration of several cell types. Two
cell types, gingival fibroblasts and periodontal ligament cells, were studied to compare
the effects of supplemental addition of PDGF, IGF-Ⅰ, TGF-β1, and EGF on
proliferation. Cells obtained from healthy human periodontal tissues were cultured in 10%
FBS supplemented with growth factor alone, and combination in the concentration of 0.1,
1, and 10 ng/㎖. Thymidine incorporation was measured after 24hours. Data from
periodontal ligament showed the following relations; in the concentration of 0.1ng/㎖
EGF>IGF-Ⅰ=PDGF=control> TGF-β1; in the concentration of 1 ng/㎖ TGF-β
1>EGF>PDGF>IGF-Ⅰ=control; in the concentration of 10 ng/㎖ TGF-β
1>PDGF>EGF>IGF-Ⅰ>control. Gingival fibroblast cultures showed the following
relations; in the concentration of 0.1 ng/㎖ TGF-β1>control>EGF=PDGF>IGF-Ⅰ ; in
the concentration of 1 ng/㎖ TGF-β1>PDGF>control>EGF>IGF-Ⅰ ; in the
concentration of 10 ng/㎖ PDGF>IGF-Ⅰ>EGF>TGF-β1 showed a significantly greater
increase in proliferation of periodontal ligament cells than in gingival fibrobalsts. In
contrast, PDGF stimulated proliferation of gingival fibroblast was significantly greater
than periondontal ligament cells. The mitotic effect of any combined growth factors
showed a significantly greater increase than that of growth factor alone, whereas the
combination of PDGF with other growth factors were not different from PDGF alone.
The data suggest that these growth factors may be valuable in promoting new
connective tissue attachment in the periodontal wound.
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